Deprenyl has been shown to slow the progression of Parkinson’s disease, (Tetrud and Langston, 1989; Parkinson Study Group, 1989), and Alzheimer’s disease (Sano et al., 1997), and is now a world-wide used drug, registered in 49 countries. In his excellent review, “Deprenyl the anti-aging psychoenergizer,” James South summarized in the Spring 2001 IAS Anti-Aging Bulletin “the unique and exciting pharmacologic/clinical profile” of this 40-year old drug that is within the capacity of everybody, (South, 2001). This paper now aims to focus attention to recent developments which throw a new light upon the anti-aging effect of deprenyl, (see Knoll, 1998, 2001, 2003 for review).
The thorough analysis of the dose-dependent effect of deprenyl on the release of catecholamines and serotonin in physiological quantities, by the aid of HPLC from isolated discrete rat brain regions, (dopamine from the striatum, substantia nigra and tuberculum olfactorium, noradrenaline from the locus coeruleus and serotonin from the raphe), revealed the existence of the enhancer regulation in the mesencephalic neurons.
We treated rats with 0.01, 0.025, 0.05, 0.1 and 0.25 mg/kg deprenyl, respectively, once daily for 21-days, isolated the discrete rat brain regions 24-hours after the last injection and measured the biogenic amines released during 20 minutes from the freshly isolated tissue samples. The amount of dopamine released from the substantia nigra and tuberculum olfactorium clarified that the dopaminergic neurons worked on a significantly enhanced activity level, even in the brain of rats treated with the lowest, 0.01 mg/kg dose of (-)-deprenyl. As this small dose of (-)-deprenyl leaves the B-type monoamine oxidase (MAO-B) activity and the uptake of amines practically unchanged, this study was the first unequivocal demonstration for the operation of a hitherto unknown enhancer mechanism in the dopaminergic neurons that is stimulated by deprenyl in very low doses, (Knoll and Miklya, 1994).
Further studies clarified the operation of the mesencephalic enhancer regulation and allowed us to realize that _-phenylethylamine (PEA), (the parent compound of deprenyl) is primarily an endogenous enhancer substance, (Knoll and Miklya, 1995; Knoll et al., 1996 a,b,c).
PEA induces in higher concentrations, a continuous strong release of the catecholamines from their intraneuronal stores, and this well known releasing effect covered up completely the enhancer effect of this trace-amine in the brain, that was classified as the prototype of the indirectly acting sympathomimetics (Knoll et al., 1996c). Amphetamine and methamphetamine, PEA derivatives with a long lasting effect, share with their parent compound the releasing property. Deprenyl was the first PEA/methamphetamine derivative that maintained the enhancer effect of its parent compounds but lost completely the releasing property. This peculiar change in the pharmacological spectrum disclosed the operation of the enhancer regulation (Knoll et al., 1996a).
We define this mechanism as follows. Endogenous mesencephalic enhancer substances capable to enhance the excitability of the mesencephalic neurons between broad limits, work in the brain, of which, for the time being, only PEA and tryptamine are the experimentally analyzed examples. Through the enhancer regulation the sensitive neurons can drastically change within a split second their activity.
This is a life important mechanism. When an eagle for example pounces upon a rabbit with lightning speed, both the attacker and the potential victim have only a split second to become properly activated. The chance for the eagle to obtain its food and for the rabbit to save its life lies in the mechanism that specific endogenous enhancer substances dynamically increase the performance of the appropriate enhancer-sensitive neurons, according to the need and the partner with the more efficiently activated brain will reach its goal, (see Knoll, 2001, 2003 for review).
The catecholaminergic and serotonergic neurons in the mesencephalon are the best models to study the enhancer regulation, as their physiologic function is to supply continuously the brain with the required amounts of catecholamines and serotonin that properly influence – activate or inhibit – billions of neurons. The significant enhancement of the nerve-stimulation induced release of [3H]-noradrenaline, [3H]-dopamine, and [3H]-serotonin from the isolated brain stem of the rat in the presence of PEA (Fig. 1) or tryptamine (Fig. 2) is shown to illustrate the mesencephalic enhancer regulation in function.
From a freshly isolated brain stem of a satisfactorily pre-treated rat a stable amount of the labeled transmitter is released for a couple of hours, (see Knoll and Miklya, 1995 for methodology). Electrical stimulation of the brain stem significantly increases the outflow of transmitters. The calculated average amount of each of the labeled transmitters released from the stimulated brain stem is the product of a surviving population of specific neurons with large individual variation in their performance.
Neurons respond to stimulation in an “all or none” manner. Hence, prior to the administration of PEA or tryptamine, only the high performing members of the population responded with transmitter release to electrical stimulation. As PEA or tryptamine enhance specifically the performance of the enhancer-sensitive neurons, the stimulation-evoked release of the labeled transmitter changed accordingly.
The data in Fig. 1 and 2 show a remarkable quantitative difference between PEA and tryptamine in their effectiveness on serotonergic neurons. A lower concentration of tryptamine, (1.3 µmol/l) proved to be much more potent than a much higher concentration of PEA (16 µmol/l) in enhancing the stimulation-evoked release of serotonin. This indicates that the enhancer regulation in the catecholaminergic and serotonergic neurons are not identical on the molecular level.
Deprenyl, the PEA-derived representative synthetic mesencephalic enhancer substance
As deprenyl was world-wide known as the first selective inhibitor of MAO-B, it was not by chance that hundreds of clinical studies with the drug were designed thereafter in the firm belief that selective blockade of MAO-B was responsible for all the effects that followed deprenyl medication. Realizing that PEA, known to be a releaser of catecholamines, is an endogenous enhancer substance and deprenyl is a PEA-derived synthetic enhancer substance devoid of the catecholamine releasing property of its parent compound, clarified that it is the enhancer effect of deprenyl that is responsible for the majority of the beneficial effects of the drug described in various experimental and clinical studies, (see Knoll, 1998, 2001 for review).
Being rapidly metabolized by MAO, PEA is short acting, and its enhancer effect can be detected in in-vitro experiments only, (see Fig. 1 and 2). As deprenyl is not metabolized, its effect is long lasting and it can reliably be measured in-vivo in a dose-dependent manner. The most convenient method for in-vivo testing of the enhancer effect of a compound is to measure the release of catecholamines and serotonin from discrete brain areas by the aid of HPLC with electrochemical detection, (see Knoll and Miklya, 1995 for details of methodology).
The subcutaneous administration of deprenyl enhanced the activity of the catecholaminergic neurons in a dose-dependent manner, (see Knoll, 2003 for review). As an example, Fig. 3 shows the enhancer effect of a single subcutaneous 0.05 mg/kg dose of deprenyl on the noradrenergic, dopaminergic and serotonergic neurons. Deprenyl is a PEA-derived enhancer substance and its in-vivo ineffectiveness on serotonergic neurons is in harmony with the finding that in the in-vitro experiments, too PEA was much less potent than tryptamine in enhancing the activity of the serotonergic neurons, (compare Fig. 1 to Fig. 2).
The tryptamine-derived representative synthetic mesencephalic enhancer substance
The discovery that tryptamine is also an endogenous enhancer substance, (Knoll, 1994) opened the way for the synthesis of a new family of enhancer compounds unrelated to PEA and the amphetamines, and R-(-)-1-(benzofuran-2-yl)-2-propylaminopentane _(-)-BPAP_ was selected as the reference compound for further studies, (Knoll et al., 1999).
According to preclinical studies, (-)-BPAP is the presently known most potent synthetic mesencephalic enhancer substance. The compound is now in the initial phase of clinical trials.
Fig. 3 shows the enhancer effect of a single subcutaneous dose of 0.0005 mg/kg of (-)-BPAP on noradrenergic, dopaminergic and serotonergic neurons. A comparison of the effect of 0.0005 mg/kg (-)-BPAP with 0.05 mg/kg deprenyl shows; (i) the substantially higher potency of (-)-BPAP than deprenyl in enhancing the activity of the catecholaminergic neurons; and (ii) the high effectiveness of (-)-BPAP on serotonergic neurons, whereas deprenyl was ineffective.
In a recent study the effect of (-)-BPAP was compared to that of the known stimulants of catecholaminergic and/or serotonergic neurons (desmethylimipramine, fluoxetine, clorgyline, lazabemide, pergolide, bromocriptine) on electrical stimulation induced release of the labeled transmitters from the isolated brain stem of rats following the incorporation of [3H]-noradrenaline or [3H]-dopamine or [3H]-serotonin by preincubation into the transmitter stores. The study confirmed the selectivity of the enhancer effect of (-)-BPAP (Miklya et al., 2003).
Fig. 4 shows the chemical structure of the two identified endogenous mesencephalic enhancer substances, PEA and tryptamine, and their synthetic derivatives, deprenyl and (-)-BPAP, used as reference compounds to study the mesencephalic enhancer regulation.
The peculiar concentration dependency of the enhancer effect
Enhancer substances stimulate the enhancer-sensitive neurons in the mesencephalon in a peculiar manner. Fig. 5 shows, as an example, the characteristics of the enhancer effect of (-)-BPAP added to isolated locus coerulei of rats. We see two bell-shaped concentration/effect curves. The one in the low nanomolar range, with a peak effect at 10-13 M concentration, is clearly demonstrating the existence of a highly sophisticated, specific form of enhancer regulation in noradrenergic neurons. The second, with a peak effect at 10-6 M concentration, shows the operation of a hundred million times less sensitive, obviously nonspecific form of the enhancer regulation in these neurons, (see Knoll et al., 2002 for details).
The great individual variation in sexual activity and learning performance in any random population of mammals of the same strain is common experience. Considering arguments speaking in favour for a key role of the enhancer regulation in the modification of behaviour through experience training or practice, (see Knoll, 2003 for review), the discovery of the bell-shaped concentration/effect curve of the enhancer substance in the low nanomolar concentration range offers a reasonable explanation for this phenomenon. To illustrate it we refer to one of our earlier studies, (Knoll et al., 1994).
In a longitudinal study performed from 1990 until 1994, we selected sexually high- and low-performing male rats from a population of 1600 rats of the same strain and tested continuously their sexual activity and their learning performance, in the shuttle box until they died. We found at the start of the experiment 99 high-performing (HP) rats that produced ejaculation in each of the four consecutive weekly mating tests used for selection, and 94 low-performing (LP) rats, unable to produce a single intromission in the four mating tests. The HP rats were significantly better performers also in the shuttle box. The striking difference in performance remained until death.
In the light of the peculiar dose-dependency of the enhancer effect shown in Fig. 5 we assume that out of the 1600 rats the 99 HP rats produced the enhancer substance inciting the sexual drive at the peak of the bell-shaped concentration/effect curve, while the 94 LP rats produced it at an inactive part of the curve; and the production of the overwhelming majority of the population (1407 rats) fall between the two extremes. The observed peculiar effect of the lifelong administration of deprenyl, the synthetic mesencephalic enhancer substance, strongly supports this conclusion.
We treated the selected HP and LP rats from the 8th month of their life three times a week, subcutaneously, with either 0.9 % NaCl or 0.25 mg/kg (-)-deprenyl until they died. Their copulatory activity was tested once a week. Their learning performance was measured in the shuttle box once in three months.
The salt-treated LP rats (N=44) never displayed ejaculation during their lifetime and were extremely dull in the shuttle box. They displayed 16.36±2.47 conditioned avoidance responses (CAR) during the first 36-week testing period. They lived 134.58±2.29 weeks. In contrast, their (-)-deprenyl-treated peers (N=48) became sexually fully active, their mating performance was substantially increased. They produced 46.85±3.91 CARs during the first 36-week testing period, and this level of performance was maintained during the 108 weeks of testing. They lived 151.24±1.36 weeks, significantly (P<0.001) longer than their salt-treated peers.
The salt-treated HP rats (N=49) displayed 14.04±0.56 ejaculations during the first 36-week testing period and due to aging they produced 2.47±0.23 ejaculation between the 73-108th weeks of testing. They displayed 78.45±3.01 CARs during the first 36-week testing period and 50.67±2.99 CARs between the 73-108th weeks of testing. They lived 152.54±1.36 weeks. In contrast, the (-)-deprenyl-treated HP rats (N=50) were significantly more active sexually than their salt-treated peers. They displayed 30.04±0.85 ejaculations during the first 36-week testing period and 7.40±0.32 ejaculation between the 73-108th weeks of testing. They were also significantly better performers in the shuttle box, produced 113.98±3.23 CARs during the first 36-week testing period and 81.68±2.14 CARs between the 73-108th weeks of testing. They lived 185.30±1.96 weeks, significantly (P<0.001) longer than their salt-treated peers.
The age-related decline of the mesenecephalic enhancer regulation and its consequences
From point of view of the anti-aging effect of the synthetic enhancer substances the peculiar age-related changes in the mesencephalic enhancer regulation deserves serious attention.
We observed already in the mid 1950s that food deprived rats in the late developmental phase of life (2 months of age) were significantly more active than those in the early post-developmental phase (4 months of age) (see Knoll, 1969 for review). In the light of our present knowledge this means that the catecholaminergic system in the mesencephalon operates at a higher activity level during the developmental phase of life. In order to verify this conclusion we revisited this problem in the mid 1990s when the HPLC technique allowed already to measure exactly the low amounts of catecholamines released from the discrete brain areas.
We measured the activity of the catecholaminergic and serotonergic neurons in the mesencephalon in groups of 2, 4, 8, 16 and 32 weeks old rats. We compared in this way the state of the enhancer regulation in rats one week before weaning (2-week old rats), one week after weaning (4-week old rats), at the age when sexual maturity was reached (8-week old rats), and in two groups in their early phase of post-developmental longevity (16- and 32-week old rats). Both in male and female rats the catecholaminergic and serotonergic neurons in the mesencephalon worked on a significantly higher level from weaning until sexual maturity, (in the 4- and 8-week old rats) than before, (2-week old rats) or after that period (16- and 32-week old rats). Rats treated any time during their post-developmental phase of life with (-)-deprenyl, showed a significantly higher catecholaminergic activity than their saline-treated peers (Knoll and Miklya, 1995).
This explains why in series of studies, rats treated during their post-developmental phase of life with deprenyl, performed better in behavioral tests, had slower age-related decline in sexual and learning performances, and lived longer than their saline-treated peers, (see Knoll, 1998, 2001 for review).
As sexual hormonal regulation starts working in the rat at the and of the 2nd month of age and we observed that the elevated level of enhancer regulation was terminated exactly at this age, it was reasonable to assume that the enhanced activity of the catecholaminergic engine of brain characteristic to the developmental period of life, was dampened by sexual hormones. To check the validity of this assumption we analyzed in detail the effect of estrone and testosterone in male and female rats. This study proved that sexual hormones terminate the hyperactive phase of adolescence by dampening the enhancer regulation, (Knoll et al., 2000). Thus, sexual maturity ends the uphill period of life and this is the beginning of post-developmental longevity with its slow continuous age-related decline until death.
Conclusion: The human catecholaminergic engine
A convincing example for the age-related decline of the catecholaminergic engine in the human brain, is the rapid decline of the dopamine content of the striatum beyond the age of 45 years. According to our present knowledge the nigrostriatal dopaminergic neurons are the most rapidly aging neurons in the human brain. The dopamine content of the human caudate nucleus decreases steeply, at a rate of 13% per decade over age 45. (Ed.- See Fig. 6) We know that symptoms of Parkinson’s disease appear if the dopamine content of the caudate sinks below 30% of the normal level. About 0.1% of the population over 40 years of age develop Parkinson’s disease, and prevalence increases sharply with age. Thus, the normal aging of the system is slow enough to avoid the appearance of Parkinsonian symptoms within the average lifespan for 99.9% of the human population.
Because of the lack of constancy of physiological age, we know that the variance within a particular age group for any measurable parameter is large. In cross-sectional studies, no single age emerges as the point of sharp decline in function. Any individual may show different levels of performance, and the careful observer finds many dissociations between “chronological” and “physiological” age. (Ed.- Antiaging advocates may refer to Professor Knoll’s “physiological” age as “biological” age). Nevertheless, the age-related decline of sexual and learning performances hits everybody on his/her own level.
Collating the results of experimental studies with clinical experiences there can be little doubt that the slow, continuous decline of the mesencephalic enhancer regulation during the post-developmental phase of life is in causal relationship with the decline of sexual and learning performances with the passing of time, and contributes to the manifestation of age-related neurological diseases. It therefore seems reasonable to enhance the activity of the catecholaminergic engine of the brain from sexual maturity until death via the administration of a safe, small daily dose of a synthetic mesencephalic enhancer substance. At present deprenyl is the only available drug for this purpose, and 1 mg is the proposed daily dose. This will work for decades. It will improve the quality of life in the latter period of life, hopefully shifting the time of natural death, with high probability decreasing the precipitation of age-related depression, eliminating or at least significantly shifting the precipitation of the symptoms of Parkinson’s disease, and possibly reducing or delaying the onset of Alzheimer’s disease, (see recent reviews, Knoll, 2001 and 2003, for details).
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